High-Level Disinfection: What, When and Why?

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High-level disinfection (HLD) is a process that eliminates all vegetative bacteria, mycobacteria, enveloped and non-enveloped viruses, fungi and some bacterial spores.

Required Level of DisinfectionEliminates
SterilisationAll forms of microbial life
High-level disinfectionVegetative bacteria, mycobacteria, enveloped and non-enveloped viruses, fungi and some bacterial spores
Intermediate-level disinfectionBacteria, mycobacteria, enveloped and non-enveloped viruses, and most fungi
Low-level disinfectionBacteria, enveloped viruses and some fungi

The Spaulding Classification determines the appropriate level of disinfection (critical, semi-critical and non-critical) for reusable medical devices. The level of disinfection required will depend on the degree of risk of infection when the device is used.1

CATEGORYLEVEL OF CONTACTDEVICE APPLICATIONREQUIRED LEVEL OF
DISINFECTION
CRITICALContact with the bloodstream or sterile tissues.Surgical instruments e.g. scalpels, tweezers, scissors, kidney dishes and clamps.Sterilisation
SEMI-CRITICALContact with mucous membranes or non-intact skin.Endoscopes and endocavity ultrasound probes.High-Level Disinfection
NON-CRITICALContact with intact skin.Skin-surface ultrasound probes


Stethoscopes and blood pressure cuffs.
Intermediate-Level Disinfection

Low-Level Disinfection

Any device which comes into contact with blood from non-intact skin or mucous membranes (including non-critical devices used in interventional procedures) classifies, as a minimum, as a semi-critical device and ultimately requires high-level disinfection or sterilisation. Occasions where this would happen to a non-critical device include:

  • Ultrasound vascular access procedures (for central line or IV placement) 
  • Biopsies 
  • Assessments where there is compromised skin or an open wound  

HLD is essential in healthcare settings to prevent healthcare-associated infections (HAIs) and combat antimicrobial resistance (AMR). HAIs can lead to serious illness, prolonged hospital stays and increased healthcare costs, often resulting from contaminated medical devices or surfaces. HLD eliminates most pathogens, including resistant organisms, reducing the risk of transmission. 


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    References:

    1 CDC Infection Control (2008). A Rational Approach to Disinfection and Sterilization. [online] CDC Infection Control. Available at: https://www.cdc.gov/infection-control/hcp/disinfectionsterilization/rational-approach.html#toc

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